Composite

Part:BBa_K1866002:Design

Designed by: Stefan Oehler   Group: iGEM15_IIT_Delhi   (2015-09-16)


Promoter+RibosomeBindingSite+NrfA+SYFP (in pSB1K3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 380
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None. This part is compatible with the 3A assembly.


Source

The biobrick BBa_K1866000 was used to obtain the Promoter+RibosomeBindingSite+NrfA construct.

Super Yellow Fluorescence Protein tag sequence was obtained from the distribution kit.

References